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Syphilis diagnostic antigen formulation of cardiolipin, cholesterol, and lecithin is optimized for sensitivity and specificity in ELISA application. Antigen is reactive with antibodies produced by patients infected with Treponemal pallidum, the causative agent of syphilis but not to anti-Treponemal antibodies. |
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Capture antigen for ELISA test to detect and measure anti-VDRL antibodies used to screen for syphilis infection and/or monitor efficacy of therapy |
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Transfer 50-75 μL of undiluted antigen solution to each well of a polystyrene ELISA plate with hydrophobic surface (e.g. Nunc PolySorp plates). Allow antigen solution to evaporate from wells until completely dry, leaving antigen bound to the test plate. Blocking plate is usually not necessary. Store plates dry with desiccant until use. Dilute blood serum/plasma specimens 1:100 into an appropriate sample diluent. Note: Traditional sample, conjugate, and wash buffer formulations will reduce or eliminate signal. For best results use Calypte Biomedical’s Sample/Conjugate Diluent D-SF (2X) (PN 93007) and Platewash PW-SF (10X) (PN 93026). Typical sample and conjugate incubation times are 30 minutes at 25-37 °C. Modify sample dilution, conjugate concentration, and incubation conditions as test results dictate. |
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Ambient |
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Ambient |
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