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Stable and reliable, non-precipitating TMB substrate (3,3',5,5'-tetramethylbenzidine) is formulated to provide color development in ELISA systems at optimal rate |
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Used as a substrate for systems employing horseradish peroxidase as the conjugate |
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Used undiluted, generally 100 μL per well following wash of enzyme conjugate from ELISA plate. Typical incubation conditions are 10-30 minutes at 25° C or 37° C depending on test. Stop reaction with 1N H2SO4 or other appropriate acidic buffer. Read plate at 450 nm. |
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2-8° C |
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Cold Packs |
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