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Solution of 1N H2SO4 in water used to stop the TMB (3,3',5,5' Tetramethylbenzidine) or OPD (o-Phenylenediamine dihydrochloride) substrate reactions in ELISA systems |
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For use as the final step in typical ELISA systems employing horseradish peroxidase as the conjugate and TMB as the substrate |
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Use undiluted, 50-100 μL per well to stop the substrate reaction |
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≤ 30° C |
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Ambient |
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